KMID : 0381120210430080987
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Genes and Genomics 2021 Volume.43 No. 8 p.987 ~ p.993
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Effects of thymosin ¥â4-derived peptides on migration and invasion of ovarian cancer cells
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Yoon Hyung-Joon
Oh Young-Lim Ko Eun-Ji Kang A-Hyun Eo Wan-Kyu Kim Ki-Hyung Lee Ji-Young Kim A-Ri Chun Sung-Wook Kim Hong-Bae Ock Mee-Sun Cha Hee-Jae
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Abstract
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Background: Thymosin ¥â4 (T¥â4) is a highly conserved actin binding protein associated with the metastatic potential of tumor cells by stimulating cell migration. The role of T¥â4 and its derived fragment peptides in migration of ovarian cancer cells has not been studied.
Objective: To analyze the effects of T¥â4 and its derived fragment peptides on ovarian cancer cell migration and invasion, we applied T¥â4 and three T¥â4-derived synthetic peptides to SKOV3 ovarian cancer cells.
Method: The migration and invasion of SKOV3 cells treated with T¥â4(1?43), T¥â4(1?15), T¥â4(12?26), T¥â4(23?), and untreated control were analyzed by in vitro migration and invasion assay with transwell plate. Cell proliferation assay was conducted to identify the effect of T¥â4 and its derived peptide on SKOV3 cell proliferation. The expression of T¥â4 related proteins related with cell proliferation was analyzed by Western blot after treatment with T¥â4 and its derived peptides.
Results: Cell migration and invasion were significantly increased in T¥â4 peptide-treated SKOV3 cells compared with untreated control. All three T¥â4-derived fragment peptides including those without an actin binding site significantly stimulated migration and invasion of SKOV3 cells. T¥â4 and its derived peptide significantly stimulated SKOV3 cell proliferation and up-regulated the expression of RACK-1 protein.
Conclusions: The T¥â4 peptide and all of its derived fragment peptides including those without an actin binding motif stimulate migration and invasion of SKOV3 ovarian cancer cells. All peptides significantly increased RACK-1 expression and cell proliferation of SKOV3 cells. These results suggest that T¥â4 stimulates migration and invasion of SKOV3 cells by stimulation of cell proliferation through up-regulation of RACK-1 protein.
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KEYWORD
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Thymosin ©¬4, Actin-binding motif, Ovarian cancer, Migration, Invasion
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